『Abstract
Vascular plants and associated microbial communities affect the
nutrient resources of terrestrial ecosystems by impacting chemical
weathering that transfers elements from primary minerals to other
ecosystem pools, and chemical denudation that transports weathered
elements out of the system in solution. We performed a year-long
replicated flow-through column growth experiment to isolate the
effects of vascular plants, ectomycorrhiza-forming fungi and associated
bacteria on chemical weathering and chemical denudation. The study
focused on Ca2+, K+ and Mg2+,
for which the sole sources were biotite and anorthite mixed into
silica sand. Concentrations of the cations were measured in input
and output solutions, and three times during the year in plant
biomass and on exchangeable cation sites of the growth medium.
Weathering and denudation fluxes were estimated by mass balance,
and mineral surface changes, biofilm and microbial attachments
to surfaces were investigated with scanning electron microscopy.
Both bacteria and fungi increased weathering fluxes compared to
abiotic controls. Without a host plant denudation rates were as
large as weathering rates i.e. the weathering to denudation ratio
was about one. Based on whole year fluxes, ectomycorrhizal seedlings
produced the greatest weathering to denudation ratios (1.5). Non-ectomycorrhizal
seedlings also showed a high ratio of 1.3. Both ectomycorrhizal
hyphal networks and root hairs of non-ectomycorrhizal trees, embedded
in biofilm (microorganisms surrounded by extracellular polymers),
transferred nutrients to the host while drainage losses were minimized.
These results suggest that biofilms localize both weathering and
plant nutrient uptake, isolating the root-hypha-mineral interface
from bulk soil solution.
Keywords: biofilm; chemical weathering; chemical denudation; column
experiment; ectomycorrhiza; root-hypha-mineral interface
Abbreviation
EMF=Ectomycorrhiza-forming fungi』
Introduction
Materials and methods
Growth medium and inoculation
Experimental design and conditions
Sample collection and analyses
Calculations and statistics
Results
Cation uptake by pine
Drainage water chemistry
Exchangeable cations
Mineral surfaces
Chemical weathering and denudation mass-balance
Discussion
Effect of vascular host on microbial and fungal weathering
and denudation
Bacterial and fungal weathering and denudation dynamics without
vascular host
Summary and conclusions
Acknowledgments
References