『Abstract
The biogeochemical alteration of an Mg-Fe orthopyroxene, reacted
for 70 yr under arid conditions in a desert environment, was studied
by transmission electron microscopy. For this purpose, an electron
transparent cross-section of the interface between a single microorganism,
an orthopyroxene and nanometer-sized calcite crystals, was prepared
with a focused ion beam system. X-ray energy dispersive spectrometry
and electron energy loss spectroscopy allowed one to clearly distinguish
the microorganism en route to fossilization from the nanometer-sized
calcite crystals, showing the usefulness of such a protocol for
identifying unambiguously traces of life in rocks. A 100-nm-deep
depression was observed in the orthopyroxene close to the microorganism,
suggesting an enhanced dissolution mediated by the microbe. However,
an Al- and Si-rich amorphous altered layer restricted to the area
just below the microorganism could be associated with decreased
silicate dissolution rates at this location, suggesting complex
effects of the microorganism on the silicate dissolution process.
The close association observed between silicate dissolution and
carbonate formation at the micrometer scale suggests that Urey-type
CO2 sequestration reactions could be mediated
by microorganisms under arid conditions.』
1. Introduction
2. Experimental methods
2.1. SEM observations and energy dispersive X-ray spectrometry
(EDXS) maps
2.2. Cross-sectioning by the FIB technique
2.3. TEM, EDXS, and electron energy loss spectroscopy (EELS)
analyses
3. Results
3.1. SEM observation of the sample
3.2. Chemistry of the calcite crystals and of the flat filament
3.3. High-resolution study of the pyroxene surface
4. Discussion
4.1. Nature of the filament
4.2. Origin of the calcites
4.3. Implications for microbial pyroxene weathering
5. Conclusion
Acknowledgments
References