『Abstract
Weathering experiments using biotite and phlogopite in the presence
of bacteria were conducted to better understand biotic dissolution
kinetics and processes (proton- and ligand-promoted dissolution)
under aerobic conditions. Miniature batch reactors (300μl in microplate
wells) were used at 24℃ for 3 days with and without bacterial
strains. Abiotic experiments were performed with organic and nitric
acids in order to calibrate the biotite-phlogopite chemical dissolution.
An empirical model was used to fit the pH dependence for iron
release rate (rFe) considering the influence
of both protons and ligands from acidic to neutral conditions
(pH ranging from 3 to 7): rFe = kH
(aH+)m +
kL (aL)l
where k is the apparent rate constant, aH+ and aL are the activities
of protons and ligands, and m and l are the reaction orders. For
both minerals in most cases at a given pH, the iron release rates
in the presence of bacteria were in good agreement with rates
determined by the chemical model and could be explained by a combination
of proton- and ligand-promoted processes. Bacteria affect mineral
dissolution and iron release rates through the quantities and
nature of the organic acids they produce. Three domains were differentiated
and proposed as biochemical models of mica dissolution: (1) below
pH 3, only proton-promoted dissolution occurred, (2) in weakly
acidic solutions both ligand- and proton-promoted mechanisms were
involved, and (3) iron immobilization occurred, at pH values greater
than 4 for biotite and greater than 5 for phlogopite. This model
allows us to distinguish the “weathering pattern phenotypes” of
strains. Bacteria that are isolated from horizons poor in carbon
appear more efficient at weathering micas than bacterial strains
isolated from environments rich in carbon. Moreover, our results
suggest that the mineral could exert a control on the release
of organic acids and the “weathering pattern phenotypes” of bacteria.』
1. Introduction
2. Materials and methods
2.1. Minerals
2.2. Bacterial strains
2.3. Experimental set-up
2.3.1. Culture media
3. Experimental procedure
3.1. Elemental analyses
3.1.1. Colorimetric determination of iron, protons and glucose
3.1.2. Metabolite release
3.2. Calculation of elemental release and dissolution rate
4. Results
4.1. Validation of the bioassay procedure
4.2. Released iron concentrations
4.2.1. Biotic experiments
4.2.2. Chemical weathering of minerals (abiotic experiments)
4.3. Iron release rates
5. Discussion
5.1. Proton-promoted versus ligand-promoted dissolution
5.2. Origin and effect of small chelating ligands (metabolic
by-products)
5.3. Iron immobilization during bacterial weathering of micas
5.4. Weathering phenotype of strains
6. Conclusion
Acknowledgments
References