『Abstract
The silicon isotope fractionation between rice plant and nutrient
solution was studied experimentally. Rice plants were grown to
maturity with the hydroponic culture in a naturally lit glasshouse.
The nutrient solution was sampled for 14 times during the whole
rice growth period. The rice plants were collected at various
growth stages and different parts of the plants were sampled separately.
The silica contents of the samples were determined by the gravimetric
method and the silicon isotope compositions were measured using
the SiF4 method.
In the growth process, the silicon content in the nutrient solution
decreased gradually from 16 mM at starting stage to 0.1-0.2 mM
at harvest and the amount of silica in single rice plant increased
gradually from 0.00013 g at start to 4.329 g at harvest. Within
rice plant the SiO2 fraction in roots reduced
continuously from 0.23 at the seedling stage, through 0.12 at
the tiller stage, 0.05 at the jointing stage, 0.023 at the heading
stage, to 0.009 at the maturity stage. Accordingly, the fraction
of SiO2 in aerial parts increased from 0.77,
through 0.88, 0.95, 0.977, to 0.991 for the same stages. The silicon
content in roots decreased from the jointing stage, through the
heading stage, to the maturity stage, parallel to the decrease
of silicon content in the nutrient solution. At the maturity stage,
the silicon content increased from roots, through stem and leaves,
to husks, but decreased drastically from husks to grains. These
observations show that transpiration and evaporation may play
an important role in silica transportation and precipitation within
rice plants
It was observed that the δ30Si of the nutrient solution
increased gradually from -0.1‰ at start to 1.5‰ at harvest, and
the δ30Si of silicon absorbed by bulk rice plant increased
gradually from -1.72‰ at start to -0.08‰ at harvest, reflecting
the effect of the kinetic silicon isotope fractionation during
silicon absorption by rice plants from nutrient solutions. The
calculated silicon isotope fractionation factor between the silicon
instantaneously absorbed by rice roots and the silicon in nutrient
solution vary from 0.9983 at start to 0.9995 at harvest, similar
to those reported for bamboo, banana and diatoms in direction
and extent. In the maturity stage, the δ30Si values
of rice organs decreased from -1.33‰ in roots to -1.98‰ in stem,
and then increased through -0.16‰ in leaves and 1.24‰ in husks,
to 2.21‰ in grains. This trend is similar to those observed in
the field grown rice and bamboo.
These quantitative data provide us a solid base for understanding
the mechanisms of silicon absorption, transportation and precipitation
in rice plants and the role of rice growth in the continental
Si cycle.』
1. Introduction
2. Rice growth experiment and analytic methods
2.1. Rice growth experiment
2.2. Sample collection and preparation
2.3. SiO2 extraction and SiO2
content determination
2.4. Silicon isotope analysis
3. Results and discussion
3.1. The silicon content of the nutrient solution and the
rice plant
3.1.1. The silicon content of the nutrient solution
3.1.2. The silica contents and distribution in different organs
of the rice plants
3.2. The silicon isotope composition of the nutrient solution
and the rice plant
3.2.1. The silicon isotope composition of the nutrient solution
3.2.2. The silicon isotope composition of the rice plants
4. Silicon isotope fractionation between rice plants and nutrient
solution
4.1. The variation of δ30Si values of bulk silicon,
dissolved silicon and gel like silica in the nutrient solution
4.2. The variation of δ30Si values of silicon absorbed
by the rice plants and silicon isotope fractionation factor between
rice plant and dissolved silicon in nutrient solution
5. Implications
5.1. Implications for the mechanism of Si uptake by rice
roots
5.2. Implications to the study of the global Si cycle
6. Conclusions
Acknowledgements
References