『Abstract
　In this study, we test the potential for passive cell wall biomineralization by determining the effects of non-metabolizing bacteria on the precipitation of uranyl, lead, and calcium phosphates from a range of over-saturated conditions. Experiments were performed using Gram-positive Bacillus subtilis and Gram-negative Shewanella oneidensis MR-1. After equilibration, the aqueous phases were sampled and the remaining metal and P concentrations were analyzed using inductively coupled plasma-optical emission spectroscopy (ICP-OES); the solid phases were collected and analyzed using X-ray diffractometry (XRD), transmission electron microscopy (TEM), and X-ray absorption spectroscopy (XAS).
　At the lower degrees of over-saturation studied, bacterial cells exerted no discernible effect on the mode of precipitation of the metal phosphates, with homogeneous precipitation occurring exclusively. however, at higher saturation states in the U system, we observed heterogeneous mineralization and extensive nucleation of hydrogen uranyl phosphate (HUP) mineralization throughout the fabric of the bacterial cell walls. This mineral nucleation effect was observed in both B. subtilis and S. oneidensis cells. In both cases, the biogenic mineral precipitates formed under the higher saturation state conditions were significantly smaller than those that formed in the abiotic controls.
　The cell wall nucleation effects that occurred in some of the U systems were not observed under any of the saturation state conditions studied in the Pb or Ca systems. The presence of B. subtilis significantly decreased the extent of precipitation in the U system, but had little effect in the Pb and Ca systems. At least part of this effect is due to higher solubility of the nanoscale HUP precipitate relative to macroscopic HUP. This study documents several effects on non-metabolizing bacterial cells on the nature and extent of metal phosphate precipitation. Each of these effects likely contributes to higher metal mobilities in geologic media, but the effects are not universal, and occur only with some elements and only under a subset of the conditions studied.』

1. Introduction
2. methods
　2.1. General approach
　2.2. Experimental methods
　　2.2.1. Bacterial preparation
　　2.2.2. Kinetics experiments
　　2.2.3. Batch precipitation experiments
　　2.2.4. Precipitation experiments using bacterial exudate solution
　　2.2.5. Biogenic mineral isolation
　　2.2.6. Solubility experiments
　2.3. Analytical methods
　　2.3.1. TEM
　　2.3.2. XRD
　　2.3.3. Synchrotron experiments
　　2.3.4. ICP-OES
　　2.3.5. TOC
　2.4. Thermodynamic modeling
　　2.4.1. Saturation states calculations
　　2.4.2. HUP solubility calculation
3. Results and discussion
　3.1. Uranium system
　　3.1.1. TEM
　　3.1.2. SAED and XRD
　　3.1.3. XAS
　　3.1.4. ICP-OES
　　3.1.5. Solubility
　　3.1.6. Effects of bacteria on uranyl phosphate precipitation
　3.2. Lead system
　　3.2.1. TEM
　　3.2.2. XRD
　　3.2.3. ICP-OES
　　3.2.4. Effect of bacteria on lead phosphate precipitation
　3.3. Calcium system
　　3.3.1. TEM
　　3.3.2. XRD
　　3.3.3. ICP-OES
　　3.3.4. Effect of bacteria on calcium phosphate precipitation
4. Conclusions
Acknowledgments
Appendix A. Supplementary data
References