『Abstract
Spatial and temporal changes in phosphorus (P) distribution,
partitioning and mobility in the benthic cyanobacterial mat (CBM)
were evaluated using sequential chemical fractionation. Total
P (TP) content was extremely low, ranging from 0.025 to 0.1 mg
g-1 DW. Exchangeable and loosely bound P, which we
consider to be mainly associated with extracellular polymeric
substances (EPS), constituted the most significant proportion
of TP (up to 52%, 55μg g-1 DW), followed by P associated
with the authigenic apatites (up to 35% of TP or 18μg g-1
DW). While we found virtually no exchange of P with the ambient
environment, our results show that the partitioning of P forms
within CBM is dependent on spatial and temporal fluctuations of
physico-chemical parameters, mainly pH and dissolved oxygen. A
conspicuous diurnal increase in the reactive, exchangeable and
loosely bound P in the top CBM layers was observed. This observation
has important ecological implications, as CBM microorganisms therefore
have an increased possibility for P “luxury” uptake during the
night. This hypothesis is further supported by the fact that P
in the organic fraction rises by as much as 53% in the upper layers
during the night, indicating some form of cellular uptake. The
P-binding potential of EPS also has ecological or biogeochemical
consequences and should be considered in stoichiometrical studies
where it represents potential danger for great overestimates of
cellular P values or the nutritional status of cells.
Keywords: Phosphorus partitioning; Diurnal changes; Sequential
fractionation; Cyanobacterial mat; EPS』
Introduction
Materials and methods
Study site and cyanobacterial mats
In situ microsensor measurements and sample collection
Cyanobacterial mat sample processing
Phosphorus fractionation
Quantification of CBM nutrient pools
Analytical methods
Results
Diurnal dynamics of DO and pH
General characteristics of the CBM cores
Phosphorus fractionation of CBM particulate matter and diurnal
changes in P partitioning
Exchangeable and loosely bound P
Phosphorus associated with authigenic apatites
Other P fractions
Quantification of diurnal changes in CBM nutrient pools
Discussion
Acknowledgment
References