Turrion,M.-B., Lafuente,F., Aroca,M.-J., Lopez(oの頭に´),O., Mulas,R. and Ruiperez(前のeの頭に´),C.(2010): Characterization of soil phosphorus in a fire-affected forest Cambisol by chemical extractions and 31P-NMR spectroscopy analysis. Science of the Total Environment, 408, 3342-3348.

『化学的抽出法と31P-NMR分光学的分析による野火の影響を受けた森林のカンビソル中の土壌リンの特徴づけ』


Abstract
 This study was conducted to investigate the long-term effects of fire on soil phosphorus (P) and to determine the efficiency of different procedures in extracting soil P forms. Different P forms were determined: labile forms (Olsen-P, Bray-P, and P extracted by anion exchange membranes: AEM-P); moderately labile inorganic and organic P, obtained by NaOH-EDTA extraction after removing the AEM-P fraction; and total organic and inorganic soil P. 31P-NMR spectroscopy was used to characterize the structure of alkali-soluble P forms (orthophosphate, monoester, pyrophosphate, and DNA). The studied area was a Pinus pinaster forest located at Arenas de San Pedro (southern Avila, Spain). The soils were Dystric Cambisols over granites. Soil samples were collected at 0-2 cm, 2-5 cm, and 10-15 cm depths, two years after a fire in the burned area and in an adjacent unburned forest area. Fire increased the total N, organic C, total P, and organic and inorganic P content in the surface soil layer. In burned soil, the P extracted by the sequential procedure (AEM and NaOH + EDTA) was about 95% of the total P. Bray extraction revealed a fire-induced increase in the sorption surfaces. Analysis by chemical methods overestimated the organic P fraction in the EDTA-NaOH extract in comparison with the determination by ignition procedure. This overestimation was more important in the burned than unburned soil samples, probably due to humification promoted by burning, which increased P sorption by soil particles. The fire-induced changes on the structure of alkali-soluble P were an increase in orthophosphate-P and a decrease in monoester-P and DNA-P.

Keywords: 31P-NMR; Forest fire; P availability; AEM-P』

1. Introduction
2. Material and methods
 2.1. Experimental site
 2.2. Soil sampling
 2.3. Soil analysis
 2.4. 31P-NMR measurements
 2.5. Statistical analysis
3. Results and discussion
 3.1. Fire effect on pH, total organic C, N, and C/N ratio
 3.2. Fire effect on total P and organic and inorganic P
 3.3. Extractable P
 3.4. Liquid-state 31P-NMR spectroscopy determination
4. Conclusions
Acknowledgements
References

※全リンの分析は、Kuo(1996)OlsenおよびBray〕による。全有機リンは、Sanders & Williams(1955)による。


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