『Abstract
The speciation of dissolved organic phosphorus (DOP) in the temperate
Tamar estuary of SW England is described. Eight stations from
the riverine to marine end-members were sampled during four seasonal
campaigns in 2007 and the DOP pool in the water column and sediment
porewater was characterized and quantified using a flow injection
manifold after sequential enzymatic hydrolysis. This enabled the
enzymatically hydrolysable phosphorus (EHP) fraction and its component
labile monoester phosphates, diester phosphates and a phytase-hydrolysable
fraction that includes myo-inositol hexakisphosphate (phytic acid),
to be determined and compared with the total DOP, dissolved reactive
phosphorus (DRP) and total dissolved phosphorus (TDP) pools. The
results showed that the DOP pool in the water column varied temporally
and spatially within the estuary (1.1-22μg L-1) and
constituted 6-40% of TDP. The EHP fraction of DOP ranged from
1.1-15μg L-1 and represented a significant and potentially
bioavailable phosphorus fraction. Furthermore the spatial profiles
of the three components of the EHP pool generally showed non-conservative
behavior along the salinity gradient, with apparent internal estuarine
sources. Porewater profiles followed broadly similar trends but
were notably higher at the marine station throughout the year.
In contrast to soil organic phosphorus profiles, the labile monoester
phosphate fraction was the largest component, with diester phosphates
also prevalent. Phytic acid concentrations were higher in the
lower estuary, possibly due to salinity induced desorption processes.
The EHP fraction is not commonly determined in aquatic systems
due to the lack of a suitable measurement technique and the Tamar
results reported here have important implications for phosphorus
biogeochemistry, estuarine ecology and the development of efficient
strategies for limiting the effects of phosphorus on water quality.』
1. Introduction
2. Materials and methods
2.1. Main characteristics of the study area
2.1.1. sampling and survey program
2.2. Procedures and analytical methods
3. Results
3.1. Dissolved reactive phosphorus (DRP), dissolved organic
phosphorus (DOP) and total enzymatically hydrolysable phosphorus
(EHP)
3.2. The different classes of DOP
4. Discussion
4.1. Dissolved organic phosphorus speciation in the Tamar
estuary
4.2. Internal cycling
4.3. Seasonal and spatial variability
4.4. Salinity gradient effects
5. Concluding remarks
Acknowledgments
References
DOP=dissolved organic phosphorus(溶存有機リン)=6-40%TDP
EHP=enzymatically hydrolysable phosphorus(酵素で加水分解可能なリン)=monoester
phosphates(主要成分)+diester phosphates+phytase(フィターゼ)-hydrolysable
phosphates(myo-inositol hexakisphosphate(phytic acid(フィチン酸))(イノシトール六リン酸分解酵素))=DOPの生物利用可能な主要成分
DRP=dissolved reactive phosphorus(溶存反応性リン)
TDP=total dissolved phosphorus(全溶存リン)