『Abstract
Historical phosphorus (P) dynamics were studied using sediment
cores from three oligotrophic, acidic lakes in Maine, USA. Long-term
oligotrophy of these lakes is consistent with high sediment aluminum
(as Al(OH)3) concentrations, as Al inhibits
internal P loading, even under reducing conditions. The role of
microbially-mediated reactions in controlling redox conditions
was evaluated by estimating microbial biomass and relative abundance
of specific functional groups. Sediments were fractionated using
a sequential chemical extraction technique and all lakes met criteria
for P retention based on threshold sediment concentrations of
Al, Fe, and P fractions as determined by (Kopacek(aの頭に´、cの頭にv)
et al. (2005) Limnol Oceanogr 52: 1147-1155). Sediment NaOH-extractable
molybdate-reactive P (rP) and non-reactive P (nrP) represent P
associated with non-reducible phases, and organic matter-related
P, respectively. Total P (TP) does not decrease with sediment
depth, as is typical of eutrophic lake sediments; however, nrP/rP
decreases and rP/TP increases for all three lakes, indicating
nrP mineralization without any significant upward diffusion and
release into the hypolimnion; i.e. diagenesis of P is conservative
within the sediment. Two diagenetic models were developed based
on nrP and rP concentrations as a function of sediment age. The
first model assumes a first-order decay of nrP, the rate coefficient
being a function of time, and represents irreversible nrP mineralization,
where the produced PO4 is permanently sequestered
by the sediment. The second model assumes a first-order reversible
biotic mineralization of organic O followed by incorporation of
inorganic P into microbial biomass. Both models reflect preservation
of TP with no loss to overlying water. The rate coefficients give
us insight into qualities of the sediment that have affected mineralization
and sequestration of phosphorus throughout the 210Pb-dateable
history of each lake. Similar models could be constructed for
other lakes to help reconstruct their trophic histories. Paleolimnological
reconstruction of the sediment P record in oligotrophic lakes
shows mineralization of nrP to rP, but unlike the case in eutrophic
lake sediments, sediment TP is preserved in these sediments.
Keywords: Lake sediment; Mineralization; Oligotrophic lake; Phosphorus;
Sediment microbes; Sequential extraction』
Introduction
Site descriptions
Materials and methods
Sediment collection and processing
Sediment chemistry
DNA extraction and quantitative PCR
Results
Sediment chemistry
Model A: Variable rate coefficient in the transformation of nrP
(the irreversible model)
Model B: Reversible reaction between nrP and rP (the reversible
model)
Microbial analysis
Discussion
Role of aluminum in lake trophic state
Transformation models
Conclusions
Acknowledgments
References
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湿った堆積物 1 g以上 | ||
1 |
1M NH4Cl、pH 7、 25℃、1時間 |
ゆるく吸着した、 交換可能な、 孔隙水画分 |
2 |
0.1M NaHCO3でバッファーした、 0.1M Na2S2O4(bicarbonate-dithionite、BD)、 40℃、30分 |
還元剤で可溶な画分〔主に、鉄とマンガンの(オキシ)水酸化物に伴うP〕 |
3 |
0.1M NaOH、 25℃、16時間 |
いくらかのアルミニウムと鉄(オキシ)水酸化物に伴うP、 いくらかの有機と生物起源P |
4 |
0.5M HCl、 25℃、16時間 |
酸溶解鉱物 |
5 |
1M NaOH、 85℃、24時間 |
残り |
AlとFeとPに対するPsenner et al. (1988)による連続抽出法の一部を変えたもの。最初のステップで、蒸留水の代わりにNH4Clを使用(Tessier et al. 1979)、第三ステップで1M NaOHの代わりに0.1M NaOHを使用(Hieltjes and Lijklema 1980)。 |