Barta（最初のaの頭に´）,J., Applova（aの頭に´）,M., Vanek（eの頭にv）,D., Kristufkova（sの頭にv、uの頭に゜、aの頭に´）,M and Santruckova（Sとcの頭にv、uの頭に゜、後のaの頭に´）,H.(2010): Effect of available P and phenolics on mineral N release in acidified spruce forest: connection with lignin-degrading enzymes and bacterial and fungal communities. Biogeochemistry, 97, 71-87.

『酸性化したトウヒ林における鉱物質窒素の放出に対する有効なリンとフェノールの影響：リグニン分解酵素および細菌と菌類の群集と関連して』

『Abstract
　We conducted over four months a short-term laboratory incubation experiment to find the best prediction parameters (i.e. initial chemical characteristics) to explain differences in microbial respiration rates and mineral N (DIN) release in different litter in an acidified spruce forest. In addition, we wanted to find the link between the activity of key extracellular ligninolytic enzymes, phoenoloxidases (PhOx) and peroxidases (Perox), microbial respiration and composition of fungal and bacterial communities. Samples of spruce needles (Pica abbes) and litter of four dominant understorey vegetation; lady fern (Athyrium alpestre), blueberry (Vaccinum myrtillus), reedgrass (Calamagrostis villosa) and hair grass (Avenella flexuosa), were collected in 2005, 2006 and 2007 from six sites located in watersheds of two glacial lakes (Plesne Lake and Certovo Lake) in the Bohemian Forest, Czech Republic. Litter samples were incubated at 0 and 10℃ in laboratory controlled conditions for 90 days. Activities of PhOx and PerOx, and C mineralization rate were measured regularly each 14 days. Litter quality characteristics and endophytic microbial community structure, based on 16SrDNA-DGGE fingerprint of bacteria and ITS-DGGE of fungi, were determined at the beginning and end of litter incubation. Our results showed a close correlation of phenolics/Pox with DIN release (r＞0.74, p＜0.001). Using multivariate analyses, Pox seems to play an important role in the change of litter fungal and bacterial community composition. At 0℃ the fungal and bacterial communities of reedgrass and blueberry litter changed in relation to Pox and Perox activity, while at 10℃ the fungal communities after the incubation were additionally affected by the phenolics/NTOT and phenolics/PTOT ratios.

Keywords: Available phosphorus; DGGE; Extracellular enzyme activities; Litter decomposition; N leaching; Phoenolics』

Introduction
Materials and methods
　Study site description
　Litter collection and experimental set-up
　Litter quality measurements
　Enzyme assays
　Extraction of DNA from litter samples and PCR amplification
　DGGE analyses
　Statistical evaluation
Results
　The role of phenolics, N and P content on litter decomposition and nutrient release
　Perox and PhOx dynamics
　Change of fungal and bacterial communities in relation to phenolics, N and P content
　　Fungal communities
　　Bacterial communities
Discussion
　The role of phenolics, N and P in litter decomposition and nutrient release
　Dynamics of Perox and PhOx activities and low temperature effect
　Dynamics of bacterial and fungal communities
Acknowledgments
References

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