『Abstract
To date, numerous studies have employed single type nitrogen
(N) addition methods in reporting influences of N deposition on
soil extracellular enzymatic activities (EEA) during litter decomposition
in forest ecosystems. As natural atmospheric N deposition is a
set of complex compounds including inorganic N and organic N,
it is essential for investigating responses of soil EEA to various
mixed N fertilization. In a subtropical forest stand in Zijin
Mountain, East China, various N fertilizers with different inorganic
N and organic N ratios were added to soils monthly from 2008 to
2009. Samples were harvested from N fertilized and control plots
every 4 months. Subsequently, six EEA were assayed. A laboratory
experiment was also conducted simultaneously. Both field and laboratory
experiments showed that various mixed N fertilizations revealed
different influences on soil EEA. Acceleration of most soil EEA
by mixed N fertilization was greater than that of single N fertilization.
The majority of soil extracellular enzymes exhibited the highest
activities under mixed N fertilization, with the ratio of inorganic
N to organic N at 3:7. These results suggested that N type and
ratio of inorganic N and organic N were important factors controlling
soil EEA, and the 3:7 ratio of inorganic N and organic N may be
the optimum for soil EEA.
Keywords: Extracellular enzyme activity; Forest soil; Inorganic
nitrogen; Nitrogen deposition; Organic nitrogen』
1. Introduction
2. Methods
2.1. Site description
2.2. Field experiment
2.3. Laboratory experiment
2.4. Chemical analysis and enzymatic assays
2.5. Statistical analyses
3. Results
3.1. Changes in soil EEA of the field experiment
3.2. Changes in soil EEA of the laboratory experiment
3.3. Changes in soil EEA and pH values within NaHCO3
addition
4. Discussion
5. Conclusion
Acknowledgements
References