wAbstract
@We conducted a greenhouse experiment to investigate the effects
of nitrogenous fertilizer application on weathered particles of
coal gob (WPCG). Different rates of nitrogenous fertilizer - low
N (0.034 g N kg-1 WPCG), medium N (0.068 g N kg-1
WPCG), and high N (0.136 g N kg-1 WPCG) - were applied,
and two native grasses (Orchardgrass and Alfalfa) were mix-planted
in each pot. The results showed that the biomass production were
all significantly increased in low and medium N group compared
with that in the control group, respectively, in aboveground or
underground part (p0.01), but it was slightly increased in high
n group. Compared with the control group, chlorophyll concentrations
of Orchardgrass in medium N group and Alfalfa in low N group were
all significantly increased (p0.05), but it was the lowest in
high N group. n uptake efficiency was the highest in low N group.
However, N, P, and K uptake efficiencies were all lowest in high
N group. Moreover, the microorganism population in WPCG was greatly
improved in low group. Compared with the control group, the numbers
of bacteria, actinomycete, fungi, and nitrogen-fixing bacteria
were significantly increased by 438, 125, 20, and 258, respectively
(p0.05). After 120 days growth of Orchardgrass and Alfalfa, the
percentage of particle diameter of WPCG was changed. The percentage
of fine particles (3 mm) was increased by 99.4. In conclusion,
it will be a more efficient approach for the quick establishment
of vegetation system to apply low nitrogenous fertilizer (0.034
g N kg-1 WPCG) as an immediate treatment for nitrogen
deficiency during the primary reclamation period on a large scale.
Keywords: Weathered particles of coal gob (WPCG); Nitrogenous
fertilizer; Biomass; Nitrogen uptake efficiency; Microorganism
populationx
1. Introduction
2. Materials and methods
@2.1. WPCG and its characteristics
@2.2. Pot experiment
@@2.2.1. Particle proportion of WPCG
@@2.2.2. Experiment design
@@2.2.3. Sampling and measurement
@2.3. Statistical analysis
3. Results
@3.1. The biomass production of Orchardgrass and Alfalfa was
increased significantly in low and medium N group
@3.2. The chlorophyll concentrations of Orchardgrass or Alfalfa
were increased in low and medium N group
@3.3. The nitrogen uptake efficiency was the highest in low N
group
@3.4. The microorganism population in WPCG was the highest in
low N group
@3.5. The percentage of particle diameter of WPCG was changed
after 120 days of grass growth
4. Discussion
5. Conclusion
Acknowledgments
References