『Abstract
　The long-term changes of ecosystem carbon (C) and nitrogen (N) pools in reclaimed mine soils remains poorly understood. Thus, age chronosequence sites, ranging from ＜1 to 25 years of reclamation, under forest and pasture land use, were identified to determine ecosystem C and N pools. Ecosystem C and N pools and selected soil properties were measured for 42v reclaimed sites across northeast Ohio. Soil organic C (SOC) was estimated by determining coal C and inorganic C, and subtracting these from the total soil C. Of the total soil C in reclaimed soil, coal C ranged from 0.2 to 15％, and inorganic C from ＜ 1 to 42％, depending on soil depth and land use. The C sequestered in the minesoil ecosystem followed a polynomial function, with the highest rate of sequestration of 6.59 Mg ha^-1 after 14 years of reclamation in the forest and 1.61 Mg ha^-1 after 6 years under pasture. After 25-year of reclamation, C sequestration was 107 Mg ha^-1 under forest ecosystem and 21 Mg ha^-1 under pasture. In the forest ecosystem, a major portion of ecosystem C consisted of soil C (94％) during the initial period (＜1 year) and biomass C (66％) during the later period (＞14 year). In the pasture ecosystem, soil C contributed a major portion of ecosystem C throughout the reclamation period: 95％ for ＜1 year and 89％ for ＞25 years of reclamation. However, soil N contributed a significant portion of ecosystem N in both the forest and pasture ecosystems. Ecosystem C and N pools and bulk densities were strongly related with reclamation age for both forest and pasture. The rates of ecosystem C and N sequestration peaked at about 10 to 15 years of reclamation. After 10 to 15 years of reclamation, the rate of sequestration decreased as reclamation age increased.

Keywords: Coal carbon; Ecosystem carbon; Ecosystem nitrogen; Inorganic carbon; Reclaimed mine soil: Soil organic carbon; Terrestrial ecosystem』

1. Introduction
2. Materials and methods
　2.1. Study area
　2.2. Soil, plant, and litter sample collection, processing, and analyses
　2.3. Assessment of ecosystem carbon and nitrogen pools and sequestration rates
　2.4. Statistical analysis
3. Results and discussions
　3.1. Physical and chemical properties of soils and its relationship with age chronosequence
　3.2. Contribution of coal and inorganic carbon in reclaimed mine soils
　3.3. Soil organic carbon and nitrogen concentrations in relation to chronosequence age
　3.4. Rate of carbon and nitrogen sequestration in terrestrial ecosystem
　3.5. Carbon and nitrogen pools in terrestrial ecosystem across chronosequences
4. Conclusions
Acknowledgements
References