Zeng,D.-H., Li,L.-J., Fahey,T.J., Yu,Z.-Y., Fan,Z.-P. and Chen,F.-S.(2010): Effects of nitrogen addition on vegetation and ecosystem carbon in a semi-arid grassland. Biogeochemistry, 98, 185-193.

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wAbstract
@To clarify responses of plant and soil carbon (C) and nitrogen (N) pools in grassland ecosystem to N addition, a field experiment was performed in a grassland in Keerqin Sandy Lands, Northeast China. We investigated vegetation composition and C and N pools of plant and soil (0-30 cm) after five consecutive years of N addition at a rate of 20 g N m-2 y-1. Vegetation composition and species diversity responded dramatically to N addition, as dominance by C4 perennials was replaced with C3 annuals. Carbon in aboveground pool increased significantly (over two-fold), mainly due to the increase of the C in aboveground living plants and surface litter, which increased by 98 and 134“, respectively. although soil C did not change significantly, the root C pool decreased in response to 5 years of N addition. The total ecosystem C pool was not significantly impacted by n addition because the large soil pool did not respond to N addition, and the increase in aboveground C was offset by the decrease in root C pool. moreover, N addition significantly increased the aboveground N pool, but had no significant effects on belowground and total ecosystem N pools. Our results suggest that in the mid-term N addition alters the C and N partitioning in above- and belowground pools, but has no significant effects on total ecosystem C and N pools in these N-limited grasslands.

Keywords: Carbon partitioning; Carbon pool; Keerqin Sandy Lands; Nitrogen limitation; Sandy grassland; Species compositionx

Introduction}
Materials and methods
@Site description
@Experimental design
@Sampling and analyses
@Data processing and analysis
Results
Discussion
@Nitrogen effects on semi-arid grassland vegetation
@Ecosystem carbon and nitrogen pools
Acknowledgments
References


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