『Abstract
Iron and phosphorus limitation and their combined effects on
the growth and development of Prorocentrum micans Ehrenberg
(Dinophyceae) and Cryptomonas sp. (Cryptophyceae) have
been studied. The growth status of these algae at different Fe
and P levels is quantitatively analyzed through lab-culture experiments.
Results show that Fe starvation can misalign chloroplast lamellas,
and metamorphose and twist thylakoid, leading to a retarded growth
rate. The combined effects of Fe and P can accelerate their growth,
and an optimum culture condition to maintain the maximum growth
rate is a combination of 0.05 μM Fe and 50 μM P. Algal uptake
of Fe and P are synergistic. Their cellular concentrations bear
significant correlations ( r = 0.9979 for Cryptomonas sp.
and 0.9938 for P.micans; P<0.01) and correspondingly the
molar P:Fe ratios are 〜160:1 and 〜356:1, respectively. These findings
will provide novel insights for revealing the crucial factors
controlling the occurrence of algal blooms.
Keywords: Iron and phosphorus limitation; Combined effect; Prorocentrum
micans Ehrenberg; Cryptomonas sp.; Algal bloom』
Introduction
Materials and methods
Results and discussion
Effect of Fe deficiency on algal growth and development
Combined effects of Fe and P on algal growth
Synergistic uptake of Fe and P
Summary
Acknowledgements
References