Rosling,A., Lindahl,B.D. ad Finlay,R.D.(2004): Carbon allocation to ectomycorrhizal roots and mycelium colonising different mineral substrates. New Phytologist, 162, 795-802.



・Ectomycorrhizal fungi occur abundantly in the mineral horizons of forest soils, but their interactions with mineral substrates are largely unknown. We have examined the proliferation of ectomycorrhizal roots and mycelium colonising different mineral substrates.
・By exposing the shoots of Pinus sylvestris seedlings to air containing 14CO2, the carbon allocation patterns in intact ectomycorrhizal associations could be monitored using electronic autoradiography.
・In plants colonised by either Hebeloma crustuliniforme or Piloderma fallax, a larger fraction of the photosynthetically derived carbon was allocated to a mineral soil substrate compared with a Sphagnum peat. /in mycorrhizal seedlings colonised by H. crustuliniforme, carbon allocation was significantly greater to roots and mycelia colonising patches of pure potassium feldspar than to those in patches of quartz.
・These results suggest that ectomycorrhizal mycelia may respond to the presence of different mineral substrates by regulating their growth and activity.

Key words: ectomycorrhiza; E horizon mineral soil; electronic autoradiography; Hebeloma crustuliniforme; potassium feldspar; Piloderma fallax, quartz』


14CO2 を含む空気にヨーロッパアカマツの実生のシュートをさらすことで、無傷の外生菌根群集における炭素配分パターンがオートラジオグラフィーを用いて監視できる。
・オオワカフサタケまたはPiloderma fallaxによりコロニーが作られた植物では、光合成から得られた炭素の大部分は、水苔と比べて鉱物土壌基質に配分された。/オオワカフサタケによりコロニーが作られた菌根実生では、石英の破片よりも純粋なカリ長石の破片にコロニーを作る根や菌糸にかなり多くの炭素が配分された。

Materials and methods
 Experimental microcosm systems
 Experiment one: Vertically divided microcosms with mineral soil and peat
 Experiment two: Peat microcosms with pure mineral patches
 14CO2-labelling, electronic autoradiography and destructive 14C analysis
 Statistical analysis
 Experiment one: Carbon allocation in vertically divided microcosms
 Experiment two: Carbon allocation in peat microcosms with pure mineral patches