Hales,H.C., Ross,D.S. and Lini,A.(2007): Isotopic signature of nitrate in two contrasting watersheds of Brush Brook, Vermont, USA. Biogeochemistry, 84, 51-66.

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wAbstract
@We used the dual isotope method to study differences in nitrate export in two subwatersheds in Vermont, USA. Precipitation, soil water and streamwater samples were collected from two watersheds in Camels Hump State Forest, located within the Green Mountains of Vermont. These samples were analyzed for the ƒΒ15N and ƒΒ18O of NO3-. The range of ƒΒN-NO3- values overlapped, with precipitation -4.5ρ to +2.0ρ (n = 14), soil solution -10.3ρ to +6.2ρ (n = 12) and streamwater +0.3ρ to +3.1ρ (n = 69). The ƒΒ18O of precipitation NO3- (mean 46.8}11.5ρ) was significantly different (Pƒ0.001) from that of the stream (mean 13.2}4.3ρ) and soil waters (mean 14.5}4.2ρ) even during snowmelt periods. Extracted soil solution and streamwater ƒΒ18O of NO3- were similar and within the established range of microbially produced NO3-, demonstrating that NO3- was formed by microbial processes. The ƒΒ15N and ƒΒ18O of NO3- suggests that although the two tributaries have different seasonal NO3- concentrations, they have a similar NO3- source.

Keywords: ƒΒ15N; ƒΒ18O; Nitrate; Nitrogen; isotopes; Oxygen isotopes; Vermontx

Introduction
Methods
@Site description
@Sample collection
@@Precipitation
@@Streamwater
@@Soil and extracted soil solution
@Laboratory analyses
@@Isotope procedure
@Quality control
@Statistical methods
Results
@Precipitation chemistry
@Streamwater chemistry
@Soil nitrate
Discussion
@Streamwater nitrate dynamics
@Streamwater nitrate sources
@Soil nitrate
@Comparison among precipitation, streamwater and soil solution
@Snow melt
Conclusions
Acknowledgements
References


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